Catherine Curie is a Research Director at IPSiM, the Institute for Plant Science of Montpellier in France. She obtained her PhD in 1992 at the University of Toulouse on the transcriptional regulation of Arabidopsis genes involved in protein translation. She went on working on the determinants of transcription through a postdoc in the lab of Prof. Sheila McCormick at UC Berkeley, focusing this time on pollen-specific regulated genes. In 1997, Catherine got a full research position at French CNRS where, first in collaboration with Dr Jean-François Briat, then as an independent group leader, she developed her project on metal homeostasis and transport in plants.
At IPSiM, her team studies the physiological and molecular mechanisms of metal ions transport in plants, through a variety of approaches including cell biology, biochemistry and molecular physiology. Among her significant contributions, we can cite the functional characterization of IRT1 and NRAMP1, the high-affinity transporters of respectively iron and manganese at the root surface of Arabidopsis, as well as the powerful histochemical method to image iron in plant tissues and cells. Catherine Curie is the author of 61 peer-reviewed articles (> 12 000 citations, H-index = 37). She has coordinated several national research grants including the 2 ANR grants “PlantMan” and “DEFIMAN” on Manganese transport, and the French-Taiwanese program “ManOmics” with Taipei Academia Sinica. She is partner of the COST European action “PLANTMETALS” involving more than 30 European Countries.
Awards:
Manganese(Mn) is an essential element for plants. As a cofactor of various enzymes and building block of biomolecules, it plays an important role in photosynthesis, production of cell walls and plasma membrane components. Besides, manganese is a cofactor of the superoxide dismutase (MnSOD) enzyme that detoxifies superoxide in the cells. Furthermore, in yeast, Mn also acts as a cytosolic antioxidant in a MnSOD-independent manner, but its mode of action remains elusive(Culotta et Daly (2013) Antioxidants & Redox Signaling: 19(9), 933-944). In plants, such function seems to be conserved since the ROS production depends on Mn level in the cytosol, which is controlled by intracellular Mn transporters(Alejandro et al (2017) The Plant Cell: 29(12), 3068-3084).
Transporters of manganese are the gate-keepers of cellular manganese and ensure the proper partitioning of manganese in the cells and organs. Perturbation of such Mn transport processes consequently affects photosynthesis efficiency,flower fertility, seed quality and response to various stress stimuli.The student will be involved in characterising the function of Mn transporters from the NRAMP and BICAT families, their interdependence and essentiality for plant processes, with special emphasis on ROS homeostasis.
Experimentally, this will involve genotyping and phenotyping of newly created transgenic lines and multiple order mutants of manganese transporters.Furthermore, the student will exploit manganese probes developed in the team indifferent mutant backgrounds and experimental conditions to localize Mn at sub-cellular level, quantify total elemental concentrations (ICP) and monitor ROS through synthetic and genetic probes. The student will therefore be involved in growing plants in vitro, and in soil,employ methods in plant genetics and molecular biology, analytical chemistry,fluorescence microscopy, luminescence imaging and image analysis.
From June 10 to September 1, 2024 (adjustable at the discretion of the organisation)